What is Gene Cloning?


complementary DNA is inserted into the vectors, or ‘naked’ DNA fragments can be taken up by directly as a host bacterium from its medium (this is less efficient than vector transfer).

Inside the host cell the recombinant DNA undergoes replication; thus, a bacterial host will give rise to a colony of cells containing the cloned target gene. Various screening methods may be used to identify such colonies, enabling them to be selected and cultured. Gene cloning facilitates gene sequencing; it also enables large quantities of a desired protein product to be produced: human insulin, for example, is now produced by bacteria containing the cloned insulin gene.


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s