What is Gene Cloning?


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complementary DNA is inserted into the vectors, or ‘naked’ DNA fragments can be taken up by directly as a host bacterium from its medium (this is less efficient than vector transfer).

Inside the host cell the recombinant DNA undergoes replication; thus, a bacterial host will give rise to a colony of cells containing the cloned target gene. Various screening methods may be used to identify such colonies, enabling them to be selected and cultured. Gene cloning facilitates gene sequencing; it also enables large quantities of a desired protein product to be produced: human insulin, for example, is now produced by bacteria containing the cloned insulin gene.

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